【摘要】 目的：建立牵牛子配方颗粒中多指标成分的含量测定方法。方法：采用HPLC结合一测多评法同时测定绿原酸等酚酸类成分的含量。色谱条件为采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5.0μm)，流动相为乙腈-0.1%磷酸，检测波长为326 nm，流速为1.0 mL·min-1，柱温为30℃，进行梯度洗脱。建立一测多评法，以咖啡酸为内参物测定牵牛子配方颗粒中酚酸类成分的含量。同时采用外标法验证本研究建立的测定方法测定结果的准确性。结果：在各自线性范围内，新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C的相对校正因子分别为1.841、1.718、1.885、1.485、1.268、1.429；一测多评法与外标法测定结果差异不显著。结论：建立的一测多评法可准确地检测牵牛子配方颗粒酚酸类多指标成分含量，作用于牵牛子配方颗粒的质量控制。更多还原

【Abstract】 Objective: To establish the quantitative analysis of multi-components in Pharbitidis formula granules. Methods: Simultaneously determine the content of phenolic acids such as chlorogenic acid using HPLC combined with one test multiple evaluation method. The chromatographic conditions are as follows:Agilent ZORBAX SB-C18(4.6 mm × 250 mm, 5.0 μ m) The mobile phase is acetonitrile 0.1 % phosphoric acid, with a detection wavelength of 326 nm, a flow rate of 1.0 mL · min-1, and a column temperature of 30 ℃ for gradient elution. Establish a one test multiple evaluation method to determine the content of phenolic acids in Pharbitidis formula granules using caffeic acid as the internal reference. Simultaneously using external standard method to verify the accuracy of the measurement results established in this study. Results: Within their respective linear ranges, the relative correction factors for neochlorogenic acid, chlorogenic acid,cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C are 1.841, 1.718, 1.885, 1.485, 1.268, and 1.429, respectively; There is no significant difference in the results between the one test multiple evaluation method and the external standard method. Conclusion: The established one test multiple evaluation method can accurately detect the content of phenolic acid multi index components in Pharbitidis formula granules, which plays a role in the quality control of Pharbitidis formula granules.更多还原